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Serologic testing is indeed the scientific report yeah retinol or other bodily excretions. along medical technology, something that generally refers to a diagnosing identifier after all specific antibody but instead allergens there in patient’s plasma. serology are being used to discover but also quantify the degrees yeah immunoglobulin as both a reaction complete pathogens, lupus, as well as interoperability through transfusion.
Importance of Serology:
Helps diagnose infectious diseases (e.g., HIV, Hepatitis, COVID-19, Syphilis).
Essential for blood typing and cross-matching in transfusions.
Assists in diagnosing autoimmune diseases (like lupus).
Plays a role in vaccine evaluation.
Used in forensic investigations and epidemiology studies.
Common techniques: ELISA, VDRL, Widal, Western blot, RPR, Agglutination, Precipitation.
Top 200 Serology MCQs with Answers
Basic Serology
What is serology primarily concerned with?
A) Hormone production
B) DNA analysis
C) Antibodies and antigens
D) RNA synthesis
Whom the biological fluids is often for use in serum going to test?
A) CSF
B) Serum
C) Urine
D) Saliva
What is the main component tested in serology?
A) WBCs
B) Platelets
C) Antibodies
D) Hemoglobin
Which test is used for the diagnosis of syphilis?
A) Widal
B) ELISA
C) VDRL
D) CRP
What does ELISA stand for?
A) Enzyme Linked Immunoglobulin Assay
B) Enzyme Linked Immunosorbent Assay
C) Enzyme Level Indicator Serum Assay
D) Electro Light Immuno Serum Analysis
Serological Techniques
Which of the following is a qualitative serological test?
A) VDRL
B) ELISA
C) Blood culture
D) PCR
The Widal test is used for diagnosing:
A) Hepatitis
B) Typhoid fever
C) HIV
D) Dengue
Agglutination tests are based on:
A) Clumping of particles
B) DNA binding
C) Cell lysis
D) None of these
Which test is used for detecting rheumatoid arthritis?
A) ASO
B) Rheumatoid Factor (RF)
C) Widal
D) CRP
The Western blot technique is used to confirm:
A) Tuberculosis
B) HIV
C) Hepatitis A
D) Dengue
Clinical Application
Which test is most sensitive for early HIV detection?
A) Western blot
B) ELISA
C) VDRL
D) RF
Hepatitis B surface antigen is tested using:
A) ELISA
B) Widal
C) VDRL
D) RPR
Which test is used to detect anti-dsDNA antibodies?
A) CRP
B) ASO
C) ELISA
D) VDRL
In dengue, which antibody appears first?
A) IgM
B) IgG
C) IgA
D) IgE
Which immunoglobulin provides long-term immunity?
A) IgM
B) IgG
C) IgA
D) IgE
Antibodies and Antigens
Which class of immunoglobulin is the largest?
A) IgG
B) IgA
C) IgM
D) IgE
Which antibody is present in allergic reactions?
A) IgA
B) IgM
C) IgE
D) IgG
What is the role of antigens in serology?
A) Produce WBCs
B) Trigger immune response
C) Form enzymes
D) None
IgA is primarily found in:
A) Blood
B) Secretions (saliva, tears)
C) Liver
D) Bones
What is seroconversion?
A) Blood clotting
B) Development of detectable antibodies
C) Antigen destruction
D) Plasma loss
Advanced Serology Tests
Which test detects the presence of CRP?
A) Widal
B) ELISA
C) Latex agglutination
D) RPR
Which disease is tested using HBsAg?
A) HIV
B) Hepatitis B
C) Dengue
D) Syphilis
RPR test is done to detect:
A) Syphilis
B) Hepatitis
C) Malaria
D) Typhoid
HIV confirmation is done by:
A) ELISA
B) Western Blot
C) Widal
D) VDRL
TORCH panel is used for:
A) Cancer
B) Prenatal infection screening
C) Malaria
D) TB
Blood Group and Transfusion Serology
Which antigen is responsible for Rh incompatibility?
A) D antigen
B) O antigen
C) A antigen
D) B antigen
In ABO blood grouping, which is a universal donor?
A) O negative
B) AB positive
C) A positive
D) B negative
Coombs test is done to detect:
A) Antibodies against RBCs
B) Malaria
C) Typhoid
D) HIV
Cross-matching is essential for:
A) Dengue detection
B) Blood transfusion
C) HIV testing
D) Widal test
Which immunoglobulin causes hemolytic disease of newborn?
A) IgG
B) IgM
C) IgA
D) IgE
Miscellaneous
Monospot test is used for:
A) Infectious mononucleosis
B) Typhoid
C) TB
D) Hepatitis
ASO test is done for:
A) HIV
B) Streptococcal infection
C) Malaria
D) Typhoid
Agglutination is:
A) RBC formation
B) Clumping of particles
C) Lysis
D) Bleeding
Positive CRP test indicates:
A) Inflammation
B) Viral fever
C) Anemia
D) Cancer
HLA typing is important for:
A) Dengue
B) Organ transplantation
C) TB
D) HIV
Test Sensitivity and Specificity
A test that correctly identifies those with disease has high:
A) Sensitivity
B) Specificity
C) Accuracy
D) Precision
Some one exam a certain properly precludes others without illness does have tall:
A) Sensitivity
B) Specificity
C) Accuracy
D) Validity
False positives are more likely when:
A) Sensitivity is high
B) Specificity is low
C) Both are high
D) None
Which test is a confirmatory test for HIV?
A) ELISA
B) Western Blot
C) PCR
D) Rapid
Which is used in quantitative ELISA?
A) Enzyme-linked color change
B) Agglutination
C) Flocculation
D) pH change
Viral & Bacterial Infections
IgM antibodies indicate:
A) Recent infection
B) Past infection
C) No infection
D) Chronic stage
Typhidot detects:
A) IgM and IgG for typhoid
B) Malaria parasite
C) Dengue antigen
D) HIV RNA
Which antibody appears late in infection?
A) IgM
B) IgG
C) IgE
D) IgA
Antibodies are produced by:
A) T cells
B) B cells
C) RBCs
D) Platelets
Autoantibodies are seen in:
A) Autoimmune diseases
B) Infections
C) Trauma
D) Cancer
More Test Applications
In COVID-19 testing, IgM shows:
A) Recent infection
B) Past exposure
C) Immunity
D) No exposure
Mantoux test is based on:
A) Delayed hypersensitivity
B) Agglutination
C) Precipitation
D) ELISA
Fluorescent antibody technique uses:
A) Fluorochrome
B) Enzymes
C) Dyes
D) Precipitates
Which test detects antistreptolysin O?
A) ASO
B) CRP
C) RF
D) Widal
HIV is diagnosed using:
A) VDRL
B) Widal
C) ELISA & Western Blot
D) Blood culture